Differential expression of selectins by mouse brain capillary endothelial cells in vitro in response to distinct inflammatory stimuli

Caroline Coisne (1) , Christelle Faveeuw (1) , Yannick Delplace (2) , Lucie Dehouck (2) , Florence Miller (1) , Roméo Cecchelli (1) , Bénédicte Dehouck (1)

1 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
2 CELLIAL Technologies

Increased lymphocyte trafficking across blood-brain barrier (BBB) is a prominent and early event in inflammatory and immune-mediated CNS diseases. The adhesion molecules that control the entry of leukocytes into the brain have not been fully elucidated. Although the role of ICAM-1 and VCAM-1 has been well documented, the expression and role of selectins is still a matter of controversy. In a mouse syngenic in vitro BBB model, highly relevant for examining immunological events, mouse brain capillary endothelial cells (MBCECs) do not express selectins. Treatment of MBCECs with LPS, induced E- and P-selectin expression, whereas TNF-alpha or IFN-gamma treatments did not. Finally, P-selectin but not E-selectin expression was induced in IL-1beta treated MBCECs. Thus, our study suggests that diverse inflammatory stimuli could differentially regulate selectin expression at the BBB.

Mots clés: E- and P-selectin expression at the blood-brain barrier; In vitro studies; Cytokines

Domaines: Biochimie, Biologie Moléculaire

Caroline Coisne, Christelle Faveeuw, Yannick Delplace, Lucie Dehouck, Florence Miller, et al.. Differential expression of selectins by mouse brain capillary endothelial cells in vitro in response to distinct inflammatory stimuli. Neuroscience Letters, 2006, 392 (3), pp.216-220. ⟨10.1016/j.neulet.2005.09.028⟩. ⟨hal-00543174⟩