Mouse syngenic in vitro blood-brain barrier model: a new tool to examine inflammatory events in cerebral endothelium

Caroline Coisne 1, 2 Lucie Dehouck 3 Christelle Faveeuw 1, 2 Yannick Delplace 3 Florence Miller 1, 2 Christophe Landry 1, 2 Céline Morissette 4 Laurence Fenart 3 Roméo Cecchelli 1, 2 Patrick Tremblay 4 Bénédicte Dehouck 1, 2
1 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
2 Institut Pasteur de Lille
3 Technologies cellulaires [Faculté Jean Perrin, Lens]
4 Neurochem inc.
Abstract : Although cerebral endothelium disturbance is commonly observed in central nervous system (CNS) inflammatory pathologies, neither the cause of this phenomenon nor the effective participation of blood- brain barrier (BBB) in such diseases are well established. Observations were mostly made in vivo using mouse models of chronic inflammation. This paper presents a new mouse in vitro model suitable for the study of underlying mechanistic events touching BBB functions during CNS inflammatory disturbances. This model consists of a coculture with both primary cell types isolated from mice. Mouse brain capillary endothelial cell (MBCEC)s coming from brain capillaries are in culture with their in vivo partners and form differentiated monolayers that retain endothelial markers and numerous phenotypic properties of in vivo cerebral endothelium, such as: (1) peripheral distribution of tight junction proteins (occludin, claudin-5, claudin-3 and JAM-1); (2) high trans-endothelium electrical resistance value; (3) attenuated paracellular flux of sucrose and inulin; (4) P-gp expression; (5) no MECA-32 expression. Furthermore, this endothelium expresses cell adhesion molecules described in vivo and shows intracellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 upregulation under lipopolysaccharide-treatment. Therefore, this well-differentiated model using autologous cells appears as a suitable support to reconstitute pathological in vitro BBB model.
keyword : P-glycoprotein Tight junction In vitro model Coculture BBB Cell adhesion molecule
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Journal articles
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Contributor : Wilfried Déplanques <>
Submitted on : Friday, December 3, 2010 - 3:46:17 PM
Last modification on : Monday, October 7, 2019 - 4:51:30 PM

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Caroline Coisne, Lucie Dehouck, Christelle Faveeuw, Yannick Delplace, Florence Miller, et al.. Mouse syngenic in vitro blood-brain barrier model: a new tool to examine inflammatory events in cerebral endothelium. Laboratory Investigation, Nature Publishing Group, 2005, 85 (6), pp.734-746. ⟨10.1038/labinvest.3700281⟩. ⟨hal-00542810⟩

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