Involvement of OCTN2 and B0,+ in the transport of carnitine through an in vitro model of the blood-brain barrier

Abstract : Carnitine is known to accumulate in brain, therefore transport of carnitine through the blood-brain barrier was studied in an in vitro system using bovine brain capillary endothelial cells (BBCEC) grown on filter inserts in a co-culture system with glial cells. Long-term exposure of BBCEC to carnitine resulted in a high accumulation of long-chain acyl carnitines, which decreased dramatically upon removal of carnitine. Kinetic analysis of carnitine accumulation indicated a possibility of functioning as more than one transporter. BBCEC were incubated in the presence of substrates and inhibitors of known carnitine transporters added from either apical or basolateral side. Inhibition by replacement of sodium and expression of OCTN2 (RT-PCR) were in agreement with Kido et al. (J. Neurochem. 2001, 79, 959–969) on the functioning of OCTN2 in apical membrane. For the first time, functioning of OCTN2 was demonstrated in the basolateral membrane, as well as functioning in both membranes of a low affinity carnitine transporter B0,+. Expression of B0,+ in BBCEC was confirmed by RT-PCR. These results suggest that OCTN2 and B0,+ could be involved in carnitine transport in both the apical and basolateral membrane.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-artois.archives-ouvertes.fr/hal-00527224
Contributor : Wilfried Déplanques <>
Submitted on : Monday, October 18, 2010 - 3:08:12 PM
Last modification on : Monday, October 18, 2010 - 3:08:12 PM

Identifiers

  • HAL Id : hal-00527224, version 1

Collections

Citation

Vincent Berezowski, Dorothea Miecz, Mariusz Marszałek, Angelika Bröer, Stefan Bröer, et al.. Involvement of OCTN2 and B0,+ in the transport of carnitine through an in vitro model of the blood-brain barrier. Journal of Neurochemistry, Wiley, 2004, 91 (4), p. 860-872. ⟨hal-00527224⟩

Share

Metrics

Record views

111