Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts

Stefan Hamm Bénédicte Dehouck 1 Jörg Kraus Karen Wolburg-Buchholz Hartwig Wolburg Werner Risau Roméo Cecchelli 2 Britta Engelhardt Marie-Pierre Dehouck 2
1 Centre de recherche Jean-Pierre Aubert-Neurosciences et Cancer
2 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
Abstract : In the central nervous system (CNS) complex endothelial tight junctions (TJs) form a restrictive paracellular diffusion barrier, the blood-brain barrier (BBB). Pathogenic changes within the CNS are frequently accompanied by the loss of BBB properties, resulting in brain edema. In order to investigate whether BBB leakiness can be monitored by a loss of TJ proteins from cellular borders, we used an in vitro BBB model where brain endothelial cells in co-culture with astrocytes form a tight permeability barrier for 3H-inulin and 14C-sucrose. Removal of astrocytes from the co-culture resulted in an increased permeability to small tracers across the brain endothelial cell monolayer and an opening of the TJs to horseradish peroxidase as detected by electron microscopy. Strikingly, opening of the endothelial TJs was not accompanied by any visible change in the molecular composition of endothelial TJs as junctional localization of the TJ-associated proteins claudin-3, claudin-5, occludin, ZO-1 or ZO-2 or the adherens junction-associated proteins β-catenin or p120cas did not change. Thus, opening of BBB TJs is not readily accompanied by the complete loss of the junctional localization of TJ proteins.
Keywords : Claudins Occludin Tight junction Edema formation Blood-brain barrier Cell culture (bovine rat)
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Article dans une revue
Cell and Tissue Research, Springer Verlag, 2004, p. 157-166
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https://hal-univ-artois.archives-ouvertes.fr/hal-00527168
Contributeur : Wilfried Déplanques <>
Soumis le : lundi 18 octobre 2010 - 14:31:53
Dernière modification le : samedi 7 avril 2018 - 14:40:02

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Stefan Hamm, Bénédicte Dehouck, Jörg Kraus, Karen Wolburg-Buchholz, Hartwig Wolburg, et al.. Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts. Cell and Tissue Research, Springer Verlag, 2004, p. 157-166. 〈hal-00527168〉

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