Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells - Archive ouverte HAL Access content directly
Journal Articles International Journal of Pharmaceutics Year : 2022

Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells

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Elisa L.J. Moya
Sonia Lombardo
Elodie Vandenhaute
  • Function : Author
Marc Schneider
  • Function : Author
Caroline Mysiorek
Akif Türeli
  • Function : Author
Takashi Kanda
  • Function : Author
Fumitaka Shimizu
  • Function : Author
Yasuteru Sano
  • Function : Author
Nathalie Maubon
  • Function : Author
Fabien Gosselet
Nazende Günday-Türeli
  • Function : Author
Marie-Pierre Dehouck
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Abstract

Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood-brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as drugs carriers are able to cross the BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs coated with PS80 and P188, labelled with a fluorescent dye were tested on human pre-clinical in vitro model to evaluate and compare their uptake profiles, mechanisms of transport and crossing over human brain-like endothelial cells (BLECs) mimicking the human BBB. In addition, these NPs were produced using a method facilitating their reproducible production at high scale, the MicroJet reactor® technology. Results showed that both formulations were biocompatible and able to be internalized within the BLECs in different uptake profiles depending on their coating: P188 NP showed higher internalization capacity than PS80 NP. Both NPs uptakes were ATP-dependent, following more than one endocytosis pathway with colocalization in the early endosomes, ending with a NPs release in the brain compartment. Thus, both surfactant-coated PLGA NPs are interesting formulations for delivery to the brain through the BBB, presenting different uptake profiles.
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Origin : Publication funded by an institution

Dates and versions

hal-03707013 , version 1 (04-07-2022)

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Elisa L.J. Moya, Sonia Lombardo, Elodie Vandenhaute, Marc Schneider, Caroline Mysiorek, et al.. Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells. International Journal of Pharmaceutics, 2022, 621, pp.121780. ⟨10.1016/j.ijpharm.2022.121780⟩. ⟨hal-03707013⟩

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