Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141) - Université d'Artois Accéder directement au contenu
Article Dans Une Revue Membranes Année : 2020

Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141)

Mira Abou-Diab
  • Fonction : Auteur
Jacinthe Thibodeau
  • Fonction : Auteur
Barbara Deracinois
Ismail Fliss
  • Fonction : Auteur
Pascal Dhulster
Naima Nedjar
  • Fonction : Auteur
Laurent Bazinet

Résumé

Neokyotorphin (α137-141) is recognized as an antimicrobial peptide and a natural meat preservative. It is produced by conventional enzymatic hydrolysis of bovine hemoglobin, a major component of cruor, a by-product of slaughterhouses. However, during conventional hydrolysis, chemical agents are necessary to adjust and regulate the pH of the protein solution and the mineral salt content of the final hydrolysate is consequently high. To produce this peptide of interest without chemical agents and with a low salt concentration, electrodialysis with bipolar membrane (EDBM), an electromembrane process recognized as a green process, with two different membrane configurations (cationic (MCP) and anionic (AEM) membranes) was investigated. Hydrolysis in EDBM showed the same enzymatic mechanism, “Zipper”, and allowed the generation of α137-141 in the same concentration as observed in conventional hydrolysis (control). EDBM-MCP allowed the production of hydrolysates containing a low concentration of mineral salts but with fouling formation on MCP, while EDBM-AEM allowed the production of hydrolysates without fouling but with a similar salt concentration than the control. To the best of our knowledge, this was the first time that EDBM was demonstrated as a feasible and innovative technology to produce peptide hydrolysates from enzymatic hydrolysis.
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Dates et versions

hal-03653924 , version 1 (18-01-2024)

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Mira Abou-Diab, Jacinthe Thibodeau, Barbara Deracinois, Christophe Flahaut, Ismail Fliss, et al.. Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141). Membranes, 2020, 10 (10), pp.257. ⟨10.3390/membranes10100257⟩. ⟨hal-03653924⟩
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