Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141) - Archive ouverte HAL Access content directly
Journal Articles Membranes Year : 2020

Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141)

, , , (1) , , , ,
1
Mira Abou-Diab
  • Function : Author
Jacinthe Thibodeau
  • Function : Author
Barbara Deracinois
Christophe Flahaut
Ismail Fliss
  • Function : Author
Pascal Dhulster
Naima Nedjar
  • Function : Author
Laurent Bazinet

Abstract

Neokyotorphin (α137-141) is recognized as an antimicrobial peptide and a natural meat preservative. It is produced by conventional enzymatic hydrolysis of bovine hemoglobin, a major component of cruor, a by-product of slaughterhouses. However, during conventional hydrolysis, chemical agents are necessary to adjust and regulate the pH of the protein solution and the mineral salt content of the final hydrolysate is consequently high. To produce this peptide of interest without chemical agents and with a low salt concentration, electrodialysis with bipolar membrane (EDBM), an electromembrane process recognized as a green process, with two different membrane configurations (cationic (MCP) and anionic (AEM) membranes) was investigated. Hydrolysis in EDBM showed the same enzymatic mechanism, “Zipper”, and allowed the generation of α137-141 in the same concentration as observed in conventional hydrolysis (control). EDBM-MCP allowed the production of hydrolysates containing a low concentration of mineral salts but with fouling formation on MCP, while EDBM-AEM allowed the production of hydrolysates without fouling but with a similar salt concentration than the control. To the best of our knowledge, this was the first time that EDBM was demonstrated as a feasible and innovative technology to produce peptide hydrolysates from enzymatic hydrolysis.

Dates and versions

hal-03653924 , version 1 (28-04-2022)

Identifiers

Cite

Mira Abou-Diab, Jacinthe Thibodeau, Barbara Deracinois, Christophe Flahaut, Ismail Fliss, et al.. Bovine Hemoglobin Enzymatic Hydrolysis by a New Ecoefficient Process—Part I: Feasibility of Electrodialysis with Bipolar Membrane and Production of Neokyotorphin (α137-141). Membranes, 2020, 10 (10), pp.257. ⟨10.3390/membranes10100257⟩. ⟨hal-03653924⟩
7 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More