Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides - Archive ouverte HAL Access content directly
Journal Articles Membranes Year : 2020

Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides

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1
Mira Abou-Diab
  • Function : Author
Jacinthe Thibodeau
  • Function : Author
Barbara Deracinois
Christophe Flahaut
Ismail Fliss
  • Function : Author
Pascal Dhulster
Laurent Bazinet
Naima Nedjar
  • Function : Author

Abstract

Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preservatives for the food sector. In this work, the hemoglobin hydrolysis to produce bioactive peptides was performed in a regulated pH medium without the use of chemical solvents and by an eco-efficient process: electrodialysis with bipolar membrane (EDBM). Bipolar/monopolar (anionic or cationic) configuration using the H+ and OH− generated by the bipolar membranes to regulate the pH was investigated. The aim of this study was to present and identify the bioactive peptides produced by EDBM in comparison with conventional hydrolysis and to identify their biological activity. The use of the EDBM for the enzymatic hydrolysis of hemoglobin has allowed for the production and identification of 17 bioactive peptides. Hydrolysates obtained by EDBM showed an excellent antimicrobial activity against six strains, antioxidant activity measured by four different tests and for the first time anti-fungal activities against five yeasts and mold strains. Consequently, this enzymatic hydrolysis carried out in regulated pH medium with bipolar membranes could provide bioactive peptides presenting antibacterial, antifungal and antioxidant interest.

Dates and versions

hal-03653921 , version 1 (28-04-2022)

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Mira Abou-Diab, Jacinthe Thibodeau, Barbara Deracinois, Christophe Flahaut, Ismail Fliss, et al.. Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides. Membranes, 2020, 10 (10), pp.268. ⟨10.3390/membranes10100268⟩. ⟨hal-03653921⟩
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