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Article Dans Une Revue Proteomics Année : 2013

Identification of additional proteins in differential proteomics using protein interaction networks

Frederik Gwinner
  • Fonction : Auteur
Adelina Acosta-Martin
  • Fonction : Auteur
Ludovic Boytard
  • Fonction : Auteur
Maggy Chwastyniak
  • Fonction : Auteur
Olivia Beseme
  • Fonction : Auteur
Hervé Drobecq
  • Fonction : Auteur
Francis Juthier
  • Fonction : Auteur
  • PersonId : 864827
Brigitte Jude
  • Fonction : Auteur
Philippe Amouyel
Florence Pinet
Benno Schwikowski
  • Fonction : Auteur

Résumé

In this study, we developed a novel computational approach based on protein-protein interaction networks to identify a list of proteins that might have remained undetected in differential proteomic profiling experiments. We tested our computational approach on two sets of human smooth muscle cell protein extracts that were affected differently by DNase I treatment. Differential proteomic analysis by saturation DIGE resulted in the identification of 41 human proteins. The application of our approach to these 41 input proteins consisted of four steps: (i) Compilation of a human protein-protein interaction network from public databases; (ii) calculation of interaction scores based on functional similarity; (iii) determination of a set of candidate proteins that are needed to efficiently and confidently connect the 41 input proteins; and (iv) ranking of the resulting 25 candidate proteins. Two of the three highest-ranked proteins, beta-arrestin 1, and beta-arrestin 2, were experimentally tested, revealing that their abundance levels in human smooth muscle cell samples were indeed affected by DNase I treatment. These proteins had not been detected during the experimental proteomic analysis. Our study suggests that our computational approach may represent a simple, universal, and cost-effective means to identify additional proteins that remain elusive for current 2D gel-based proteomic profiling techniques.

Dates et versions

hal-02515838 , version 1 (23-03-2020)

Identifiants

Citer

Frederik Gwinner, Adelina Acosta-Martin, Ludovic Boytard, Maggy Chwastyniak, Olivia Beseme, et al.. Identification of additional proteins in differential proteomics using protein interaction networks. Proteomics, 2013, 13 (7), pp.1065-1076. ⟨10.1002/pmic.201200482⟩. ⟨hal-02515838⟩
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