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Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses

Abstract : In most proteomics analyses and in particular for the “off-gel” approaches, based essentially on chromatography, the complexity of the proteome should be reduced; otherwise identifications can be hindered, especially if the mass spectrometry analysis is not conducted using state-of-the-art instrumentation. Even if the method used is a bottom-up proteomics, it appears mandatory to pre-fractionate the proteins in order to reduce the complexity. We report here the development and validation of a pre-fractionation based on the differential solubilisation of proteins using increasing concentrations of acetonitrile (ACN). This “ACN fractionation” was applied to the study of the Triton X-100 soluble sub-proteome of brain capillary endothelial cells (BCEC) with re-induced blood-brain barrier (BBB) functions.
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https://hal-univ-artois.archives-ouvertes.fr/hal-02511059
Contributor : Virginie Justin-Labonne <>
Submitted on : Wednesday, March 18, 2020 - 2:35:06 PM
Last modification on : Thursday, October 15, 2020 - 5:54:04 PM

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Yannis Karamanos, Barbara Deracinois, Gwënaël Pottiez, Sophie Duban-Deweer. Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses. OALib Journal, Open Access Library 2015, 02, ⟨10.4236/oalib.1101928⟩. ⟨hal-02511059⟩

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