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Journal articles
Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses
Abstract : In most proteomics analyses and in particular for the “off-gel” approaches, based essentially on chromatography, the complexity of the proteome should be reduced; otherwise identifications can be hindered, especially if the mass spectrometry analysis is not conducted using state-of-the-art instrumentation. Even if the method used is a bottom-up proteomics, it appears mandatory to pre-fractionate the proteins in order to reduce the complexity. We report here the development and validation of a pre-fractionation based on the differential solubilisation of proteins using increasing concentrations of acetonitrile (ACN). This “ACN fractionation” was applied to the study of the Triton X-100 soluble sub-proteome of brain capillary endothelial cells (BCEC) with re-induced blood-brain barrier (BBB) functions.
https://hal-univ-artois.archives-ouvertes.fr/hal-02511059
Contributor : Virginie Justin-Labonne Connect in order to contact the contributor
Submitted on : Wednesday, March 18, 2020 - 2:35:06 PM
Last modification on : Friday, January 21, 2022 - 3:30:01 AM
Contributor : Virginie Justin-Labonne Connect in order to contact the contributor
Submitted on : Wednesday, March 18, 2020 - 2:35:06 PM
Last modification on : Friday, January 21, 2022 - 3:30:01 AM