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In vitro models of the blood–brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use

Hans Helms 1 N Joan Abbott 2 Malgorzata Burek 3 Roméo Cecchelli 4 Pierre-Olivier Couraud 5 Mária Deli 6 Carola Förster 3 Hans Galla 7 Ignacio Romero 8, 9 Eric Shusta 10 Matthew Stebbins 10 Elodie Vandenhaute 4 Babette Weksler 11 Birger Brodin 1
1 KU - University of Copenhagen = Københavns Universitet
2 King‘s College London
3 University of Würzburg
4 LBHE - Laboratoire de la Barrière Hémato-Encéphalique
5 IC UM3 (UMR 8104 / U1016) - Institut Cochin
6 Institute of Biophysics
BRC - Biological Research Centre [Budapest]
7 University of Muenster
8 Department of Life, Health and Chemical Sciences
9 OU - The Open University [Milton Keynes]
10 University of Wisconsin-Madison
11 Division of Hematology and Oncology
Abstract : The endothelial cells lining the brain capillaries separate the blood from the brain parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial interface for exchange of nutrients, gases, and metabolites between blood and brain, and as a barrier for neurotoxic components of plasma and xenobiotics. This "blood-brain barrier" function is a major hindrance for drug uptake into the brain parenchyma. Cell culture models, based on either primary cells or immortalized brain endothelial cell lines, have been developed, in order to facilitate in vitro studies of drug transport to the brain and studies of endothelial cell biology and pathophysiology. In this review, we aim to give an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. As an ideal cell culture model of the blood-brain barrier is yet to be developed, we also aim to give an overview of the advantages and drawbacks of the different models described.
Keywords : Blood–brain barrier astrocytes endothelium pericytes stem cells
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https://hal-univ-artois.archives-ouvertes.fr/hal-02510167
Contributor : Virginie Justin-Labonne <>
Submitted on : Tuesday, March 17, 2020 - 3:03:07 PM
Last modification on : Thursday, April 9, 2020 - 11:52:54 AM

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Hans Helms, N Joan Abbott, Malgorzata Burek, Roméo Cecchelli, Pierre-Olivier Couraud, et al.. In vitro models of the blood–brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2016, 36 (5), pp.862-890. ⟨10.1177/0271678X16630991⟩. ⟨hal-02510167⟩

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