Cross-talk between brain capillary endothelial cells and astrocytes: upregulation of the low density lipoprotein receptor at the blood-brain barrier

Bénédicte Dehouck 1 Laurence Tilloy-Fenart 2 Marie-Pierre Dehouck 2 Roméo Cecchelli 2 Jean-Charles Fruchart
1 JPArc - U837 Inserm - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer
2 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
Abstract : In contrast to the endothelial cells in large vessels where low density lipoprotein receptors are down-regulated, brain capillary endothelial cells in vivo express an LDL receptor. Using a cell culture model of the blood-brain barrier consisting of a coculture of brain capillary endothelial cells and astrocytes, we observed that the capacity of endothelial cells to bind LDL is enhanced 3 fold when cocultured with astrocytes. We next investigated the ability of astrocytes to modulate endothelial cell LDL receptor expression. We have shown that the lipid requirement of astrocytes increases the expression of endothelial cell LDL receptors. Experiments with dialysis membranes of different pore size showed that this effect is mediated by a soluble factor(s) with a molecular mass somewhere between 3,500 and 14,000. Substituting astrocytes with smooth muscle cells or brain endothelium with endothelium from the aorta or the adrenal cortex did not enhance the luminal LDL receptor expression on endothelial cells, demonstrating the specificity of the interactions. This study demonstrates that a cross-talk takes place between brain capillary endothelial cells and astrocytes.
Keywords : Blood-brain barrier LDL receptor Endothelial cells Upregulation
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Journal articles
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Contributor : Wilfried Déplanques <>
Submitted on : Tuesday, December 14, 2010 - 4:34:23 PM
Last modification on : Saturday, April 7, 2018 - 2:40:02 PM

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Bénédicte Dehouck, Laurence Tilloy-Fenart, Marie-Pierre Dehouck, Roméo Cecchelli, Jean-Charles Fruchart. Cross-talk between brain capillary endothelial cells and astrocytes: upregulation of the low density lipoprotein receptor at the blood-brain barrier. Nutrition, Metabolism and Cardiovascular Diseases, Elsevier, 1997, 7, p. 225-230. ⟨hal-00546733⟩

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