Peroxisome proliferator-activated receptor-α activation protects brain capillary endothelial cells from oxygen-glucose deprivation-induced hyperpermeability in the blood-brain barrier

Abstract : That promising neuroprotectants failed to demonstrate benefit against stroke highlights the great difficulties to translate preclinical pharmacological effects in clinical outcomes. Part of this hurdle implies the complex response to injury of the neurovascular unit increasing the cerebrovascular permeability at the level of the blood-brain barrier (BBB). Previous studies reported neuroprotection in animal models upon activation of the nuclear receptor PPARα (peroxisome proliferator-activated receptor) α, but the cellular targets at the BBB level remain largely unexplored. Here, to study whether PPAR-α activation acts on BBB permeability, we adapted a mouse BBB cell model to ischaemic conditions at the stage of occlusion defined in vitro as oxygen-glucose deprivation (OGD). This model consists of a co-culture of brain capillary endothelial cells (ECs) on a filter insert placed upon a rat glial cell culture. The EC monolayer permeability increase induced by 4 h of OGD was significantly restricted after treatment with the PPAR-α agonist fenofibric acid (FA) 24 h before or at the onset of OGD. Treatments of separated ECs or glial cells showed that this protective effect was conferred by BBB ECs but not glial cells. Furthermore, co-cultures with ECs from PPAR-α-deficient mice revealed that FA had no effect on OGD-induced hyperpermeability. No transcriptional modulation of classical PPAR-α target genes such as SOD, ICAM-1, VCAM-1, ACO, CPT-1, PDK-4 or ET-1 was observed in wild type mouse ECs. In conclusion, these results suggest that part of the preventive PPAR-α-mediated protection may occur via BBB ECs by limiting hyperpermeability.
Type de document :
Article dans une revue
Current Neurovascular Research, Bentham Science Publishers, 2009, 6, p. 181-193
Liste complète des métadonnées

https://hal-univ-artois.archives-ouvertes.fr/hal-00544019
Contributeur : Wilfried Déplanques <>
Soumis le : mardi 7 décembre 2010 - 10:20:52
Dernière modification le : jeudi 26 avril 2018 - 01:04:57

Identifiants

  • HAL Id : hal-00544019, version 1

Collections

Citation

Caroline Mysiorek, Maxime Culot, Lucie Dehouck, Bruno Derudas, Bart Staels, et al.. Peroxisome proliferator-activated receptor-α activation protects brain capillary endothelial cells from oxygen-glucose deprivation-induced hyperpermeability in the blood-brain barrier. Current Neurovascular Research, Bentham Science Publishers, 2009, 6, p. 181-193. 〈hal-00544019〉

Partager

Métriques

Consultations de la notice

94