A polarized localization of amino acid/carnitine transporter B<SUP>0,+</SUP> (ATB<SUP>0,+</SUP>) in the blood-brain barrier - Archive ouverte HAL Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 2008

A polarized localization of amino acid/carnitine transporter B0,+ (ATB0,+) in the blood-brain barrier

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Abstract

Brain capillary endothelial cells control the uptake and efflux from the brain of many hydrophilic compounds due to highly specialized transporters often localized in a polarized way. Localization of Na+- and Cl--dependent amino acid and carnitine transporter B0,+ (ATB0,+) was studied in a co-culture of bovine brain capillary endothelial cells (BBCEC) grown on filters above astrocytes (an in vitro blood-brain barrier model). Immunoblotting and three-dimensional immunocytochemistry analysis with anti- B0,+antibodies demonstrated the presence of this transporter and its prevalent co-localization with P-glycoprotein i.e. at the apical side. The sensitivity of leucine uptake through the apical membrane to 2-aminobicyclo-[2.2.1]-heptane-2-carboxylic acid (BCH), D-serine as well as sodium and chloride replacement confirm the functioning of ATB0,+ and suggests an important physiological role of ATB0,+ in controlling the delivery of amino acids and carnitine to the brain.
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Dates and versions

hal-00543646 , version 1 (06-12-2010)

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  • HAL Id : hal-00543646 , version 1

Cite

Magdalena Czeredys, Caroline Mysiorek, Natalia Kulikova, Lukasz Samluk, Vincent Berezowski, et al.. A polarized localization of amino acid/carnitine transporter B0,+ (ATB0,+) in the blood-brain barrier. Biochemical and Biophysical Research Communications, 2008, 376 (2), p. 267-270. ⟨hal-00543646⟩

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