Indirect evidence that drug brain targeting using polysorbate 80-coated polybutylcyanoacrylate nanoparticles is related to toxicity

Jean-Christophe Olivier 1 Laurence Fenart 2 Romain Chauvet Claudine Pariat Roméo Cecchelli 2 William Couet 1, 3, 4
1 Pharmacologie des anti-infectieux
2 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
3 Université de Poitiers - Faculté de Médecine et de Pharmacie
4 CHU Poitiers - Centre hospitalier universitaire de Poitiers
Abstract : Purpose. To investigate the mechanism underlying the entry of the analgesic peptide dalargin into brain using biodegradable polybutylcyanoacrylate (PBCA) nanoparticles (NP) overcoated with polysorbate 80. Methods. The investigations were carried out with PBCA NP and with non biodegradable polystyrene (PS) NP (200 nm diameter). Dalargin adsorption was assessed by HPLC. Its entry into the CNS in mice was evaluated using the tail-flick procedure. Locomotor activity measurements were performed to compare NP toxicities. BBB permeabilization by PBCA NP was studied in vitro using a coculture of bovine brain capillary endothelial cells and rat astrocytes. Results. Dalargin loading was 11.7 μg/mg on PBCA NP and 16.5 μg/ mg on PS NP. Adding polysorbate 80 to NP led to a complete desorption. Nevertheless, dalargin associated with PBCA NP and polysorbate 80 induced a potent and prolonged analgesia, which could not be obtained using PS NP in place of PBCA NP. Locomotor activity dramatically decreased in mice dosed with PBCA NP, but not with PS NP. PBCA NP also caused occasional mortality. In vitro, PBCA NP (10 μg/ml) induced a permeabilization of the BBB model. Conclusions. A non specific permeabilization of the BBB, probably related to the toxicity of the carrier, may account for the CNS penetration of dalargin associated with PBCA NP and polysorbate 80.
Keywords : Polybutylcyanoacrylate Polystyrene Nanoparticles Blood-brain barrier Dalargin Brain targeting
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Journal articles
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https://hal-univ-artois.archives-ouvertes.fr/hal-00534822
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Submitted on : Wednesday, November 10, 2010 - 3:36:08 PM
Last modification on : Wednesday, May 15, 2019 - 10:30:04 AM

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Jean-Christophe Olivier, Laurence Fenart, Romain Chauvet, Claudine Pariat, Roméo Cecchelli, et al.. Indirect evidence that drug brain targeting using polysorbate 80-coated polybutylcyanoacrylate nanoparticles is related to toxicity. Pharmaceutical Research / Pharmaceutical Research (Dordrecht), 1999, 16 (12), p. 1836-1842. ⟨hal-00534822⟩

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