Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries - Archive ouverte HAL Access content directly
Journal Articles Journal of Cerebral Blood Flow and Metabolism Year : 1997

Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries

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Abstract

Endothelial cells and pericytes are closely associated in brain capillaries. Together with astrocytic foot processes, they form the blood-brain barrier. Capillaries were isolated from bovine brain cortex. Pure populations of endothelial cells and pericytes were isolated and cultured in vitro. Polarized monolayers of endothelial cells preferentially secreted immunoreactive endothelin-1 (Et-1) at their abluminal (brain-facing) membrane. They did not express receptors for Et-1. Pericytes expressed BQ-123-sensitive ETA receptors for endothelins as evidenced by 125I-Et-1 binding experiments. These receptors were coupled to phospholipase C as demonstrated by intracellular calcium measurements using indo-1-loaded cells. Addition of Et-1 to pericytes induced marked changes in the cell morphology that were associated with a reorganization of F-actin and intermediate filaments. It is concluded that Et-1 is a paracrine mediator at the bovine blood-brain barrier and that capillary pericytes are target cells for endothelium-derived Et-1.
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Dates and versions

hal-00524699 , version 1 (08-10-2010)

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  • HAL Id : hal-00524699 , version 1

Cite

Marie-Pierre Dehouck, Paul Vigne, Gérard Torpier, Jean-Philippe Breittmayer, Roméo Cecchelli, et al.. Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries. Journal of Cerebral Blood Flow and Metabolism, 1997, 17, p. 464-469. ⟨hal-00524699⟩
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