Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries

Marie-Pierre Dehouck 1 Paul Vigne 2 Gérard Torpier Jean-Philippe Breittmayer 3 Roméo Cecchelli 1 Christian Frelin 2
1 LBHE - Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique
2 ISBDC - Institut de signalisation, biologie du développement et cancer
3 Régulations des réactions immunitaires et inflammatoires
Abstract : Endothelial cells and pericytes are closely associated in brain capillaries. Together with astrocytic foot processes, they form the blood-brain barrier. Capillaries were isolated from bovine brain cortex. Pure populations of endothelial cells and pericytes were isolated and cultured in vitro. Polarized monolayers of endothelial cells preferentially secreted immunoreactive endothelin-1 (Et-1) at their abluminal (brain-facing) membrane. They did not express receptors for Et-1. Pericytes expressed BQ-123-sensitive ETA receptors for endothelins as evidenced by 125I-Et-1 binding experiments. These receptors were coupled to phospholipase C as demonstrated by intracellular calcium measurements using indo-1-loaded cells. Addition of Et-1 to pericytes induced marked changes in the cell morphology that were associated with a reorganization of F-actin and intermediate filaments. It is concluded that Et-1 is a paracrine mediator at the bovine blood-brain barrier and that capillary pericytes are target cells for endothelium-derived Et-1.
Keywords : Blood-brain barrier Cytoskeleton BQ-123 ETA receptor Polarity
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Article dans une revue
Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 1997, 17, p. 464-469
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Contributeur : Wilfried Déplanques <>
Soumis le : vendredi 8 octobre 2010 - 15:42:32
Dernière modification le : jeudi 3 mai 2018 - 13:32:03

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Marie-Pierre Dehouck, Paul Vigne, Gérard Torpier, Jean-Philippe Breittmayer, Roméo Cecchelli, et al.. Endothelin-1 as a mediator of endothelial cell-pericyte interactions in bovine brain capillaries. Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 1997, 17, p. 464-469. 〈hal-00524699〉

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