Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Astrocytes are mainly responsible for the polyunsaturated fatty acid enrichment in blood–brain barrier endothelial cells in vitro

Abstract : To determine the respective roles of endothelial cells from brain capillaries and astrocytes in the conversion of circulating 18:2n–6 and 18:3n–3 into 20:4n–6 and 22:6n–3, respectively, a coculture of the two cell types mimicking the in vivo blood–brain barrier was used. During the culture period, endothelial cells cultured on an insert were set above the medium of a Petri dish containing or not a stabilized culture of astrocytes. Five days after confluence, labeled 18:2n–6 and 18:3n–3 (10 μΜ each) were added to the endothelial cells and incubated for 48 h. Analogous experiments were also performed by using each cell type cultured alone in the culture device. The distribution of radioactivity in lipids and fatty acids was studied in all the compartments of the culture device. Endothelial cells cultured alone weakly converted the precursor fatty acids into 20:4n–6 and 22:6n–3. When endothelial cells were cocultured with astrocytes, their content of polyunsaturated fatty acids increased dramatically. This effect was associated with the uptake of polyunsaturated fatty acids from the lower medium (astrocyte medium). These fatty acids were released by astrocytes after they were synthesized from the precursor fatty acids that passed through the endothelial cell monolayer into the lower medium. Polyunsaturated fatty acids were released by astrocytes as unesterified fatty acids and as phospholipids (mainly phosphatidylcholine and lysophosphatidylcholine) even when the medium was devoid of serum. These results suggest that astrocytes could play a major role in the delivery of essential polyunsaturated fatty acids to the barrier itself and to the brain.
Document type :
Journal articles
Complete list of metadata
Contributor : Wilfried Déplanques Connect in order to contact the contributor
Submitted on : Wednesday, October 6, 2010 - 4:28:02 PM
Last modification on : Wednesday, July 6, 2022 - 9:15:02 AM


  • HAL Id : hal-00523952, version 1


N. Bernoud, C. Bénistant, J.F. Pageaux, Marie-Pierre Dehouck, P. Molière, et al.. Astrocytes are mainly responsible for the polyunsaturated fatty acid enrichment in blood–brain barrier endothelial cells in vitro. Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 1998, 39, p. 1816-1824. ⟨hal-00523952⟩



Record views