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A simple ATAC-seq protocol for population epigenetics

Abstract : We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise.
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https://hal.archives-ouvertes.fr/hal-03145575
Contributor : Anne Modat <>
Submitted on : Thursday, February 18, 2021 - 2:40:21 PM
Last modification on : Tuesday, March 30, 2021 - 12:26:18 PM

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Ronaldo de Carvalho Augusto, Olivier Rey, Céline Cosseau, Cristian Chaparro, Jérémie Vidal-Dupiol, et al.. A simple ATAC-seq protocol for population epigenetics. Wellcome Open Research, F1000Research, 2021, 5, pp.121. ⟨10.12688/wellcomeopenres.15552.2⟩. ⟨hal-03145575⟩

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